INTRODUCTION
Microsporidian disease in shrimp is caused by the parasite Enterocytozoon Hepatopenaei (EHP). They live parasitically in hepatopancreatic epithelial cells, hindering the absorption of nutrients, causing stunted shrimp growth. The disease is detected in both black tiger shrimp and white leg shrimp and can spread through food or from sick shrimp to healthy shrimp.
TopSPEC® Air-Dryable EHP qPCR Kit is a test kit that detects the presence of Enterocytozoon Hepatopenaei (EHP) (SWP1 gene) on shrimp samples using Real-time PCR technique. The kit helps detect pathogens present in the farming environment early and quickly. In addition, with the use of dry mix technology, the kit can be stored at cool temperatures, convenient for direct on-site testing, saving time and effort in transporting samples to the laboratory.
TopSPEC® Air-dryable EHP qPCR Kit
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours.
- Various input sample types
- High specificity and sensitive
- It can be stored at cool temperatures, convenient for on-site testing
Specification
| Target | Enterocytozoon Hepatopenaei (EHP) – SWP1 gene |
| Sample type | Shrimp tissue samples: gills, swimming feet, liver, pancreas, stomach, intestinal tract |
| Extraction sample volume | 200 µL |
| DNA volume for qPCR | 20 µL |
| Color channel detection | FAM: detect EHP HEX: internal control |
| Technology | TaqMan probe |
| PCR time | 1h30m |
| Specificity | Only EHP is detected |
| Component | EHP qPCR mix tube, Negative control, Positive control, Internal control (IC), TE 1X |
| Storage | 12 months at 2-8oC |
Note: Tube contains EHP qPCR mix, positive control, internal control in dry form; Positive and internal control tubes were reconstituted with TE 1X according to the volume stated on the label. After reconstitution, the product is stored at -20oC.
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