INTRODUCTION
Malaria is a common infectious disease caused by the Plasmodium spp. Symptoms and signs include fever (periodic) chills, sweating, hemolytic anemia, and enlarged spleen. In 2017, there were approximately 219 million cases of malaria worldwide, resulting in 435,000 deaths, primarily in children under 5 years old in Africa.
TopSENSI® Plasmodium spp. qPCR Kit is a testing kit based on Real-time PCR method that allows for the rapid and accurate detection of the malaria-causing agent in whole blood samples and patient DBS samples. This kit optimizes the time for the entire process, offering high sensitivity and specificity.
TopSENSI® Plasmodium spp. qPCR Kit
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours
- High specificity and sensitivity
- Taqman probe technology
Specification
| Target | Plasmodium spp. |
| Sample type | Whole blood sample, DBS |
| Input sample volume | 200µL |
| DNA volume | 10µL |
| Color channel detection | FAM: detect pathogen
HEX: internal control |
| Technology | TaqMan probe |
| PCR time | 1h30m |
| Specificity | Only detect Plasmodium spp. |
| Component | qPCR mix, Negative control, Positive control, Internal control (IC), Tube PCR. |
| Storage | 12 months at -20oC |
Recommended extraction kit
| Silica column extraction | HI-112
HI-132 |
TopPURE® Genomic DNA Extraction Kit |
