INTRODUCTION
Monkeypox disease is a dangerous infectious illness caused by the Monkeypox virus, which belongs to the Orthopox virus genus within the Poxviridae family. Currently, Monkeypox disease is spreading and escalating in many countries in Africa and Europe, with highly complex developments.
TopSENSI® MPXV qPCR KIT is a Real-time PCR testing kit that enables rapid and accurate detection of the Monkeypox virus in surface skin swab samples. The kit offers several notable advantages, including a simple and quick procedure, high sensitivity, and high specificity.
TopSENSI® MPXV qPCR KIT
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours
- High specificity and sensitivity
- Taqman probe technology
Specification
| Target | Monkeypox virus |
| Input sample | samples from secretions, surface swabs |
| Input sample volume | 200 µL |
| DNA volume | 10 µL |
| Quantitative detection | FAM: detect MPXV
HEX: internal control Rnase P |
| Fluorescent channel | TaqMan probe |
| PCR time | 1h30m |
| Sensitivity | 3.85 copies/reaction |
| Specificity | Only detect MPXV |
| Storage | 12 months at -20oC |
| Components of | MPXV qPCR mix, negative control, positive control, internal control, tube PCR |
Recommended extraction kit
| Silica column extraction | HI-112
HI-712 |
TopPURE® Genomic DNA Extraction Kit |
| Automatic extraction | HI-612
HI-007 |
TopPURE® Maga Genomic DNA/RNA Extraction Kit |
