Introduction
Herpes simplex virus 1-2 (HSV1-2) is a prevalent virus in humans belonging to the Herpesviridae family, Alphaherpesvirinae subfamily, rapidly transmitted through direct mucosal contact between individuals. Most early cases of HSV1-2 infection are asymptomatic, and as the disease progresses, symptoms may manifest, such as the appearance of ulcers, blisters around the mouth, and genital areas. In pregnant women, HSV1-2 infection during pregnancy can be transmitted from mother to infant, leading to neonatal herpes. Additionally, persistent HSV1-2 infection can result in various neurological disorders such as meningitis, encephalitis, nerve degeneration, and ocular inflammation. Therefore, accurate detection and identification of HSV1-2 play a crucial role in treatment and prevention efforts.
TopSENSI® HSV1-2 Multiplex qPCR Kit is a testing kit designed for the detection of the presence of DNA-HSV1 and DNA-HSV2 in samples extracted from various specimens from human using the Real-time PCR technique. The test results from this diagnostic test kit are solely intended for clinical reference and should not be utilized as the exclusive basis for diagnosis or exclusion.
TopSENSI® HSV1-2 Multiplex qPCR Kit (undivided master mix)
Highlights
- Simple procedure
- Optimize the PCR time to 1.5 hours
- High specificity and sensitivity
- Taqman probe technology
Specification
| Target | Herpes simplex virus 1-2 (HSV1-2) |
| Sample type | Vaginal/Urethral swab samples and serum samples |
| Input sample volume | 200µL |
| DNA volume | 10µL |
| Color channel detection | FAM: detect HSV1 Texas Red: detect HSV2 HEX: internal control |
| Analytical sensitivity | 5,00 – 15,00 copies/reaction |
| Technology | TaqMan probe |
| PCR time | 1h30m |
| Specificity | The kit specifically detects HSV1-2 |
| Component | HSV1-2 qPCR mix, negative control, positive control, internal control(IC), Tube PCR. |
| Storage | 12 months at -20oC |
Recommended extraction kit
| Silica column extraction | HI-112 | TopPURE® Genomic DNA Extraction Kit |
| Automatic extraction | HI-612
HI-007 |
TopPURE® Maga Genomic DNA/RNA Extraction Kit |
