INTRODUCTION
TopSENSI® GBS qPCR Kit is a test kit based on the real-time PCR method that allows fast and accurate detection of GBS agent from vaginal swab samples. The kit has the advantages of specificity, high sensitivity, endogenous control is available to help control the extraction process or master mix.
Group B Streptococcus (GBS) is a gram-positive bacterium, commonly found in pregnant women ‘s vagina and rectum. Women infected with GBS are usually ineffective and harmless, but in some cases can cause infection of the cervix. For pregnant women, during pregnancy, GBS infection can lead to neonatal infection in the baby, if progression severe, it can lead to death. Therefore, the screening and detection of this bacterium before birth is an extremely urgent problem.
ADVANTAGES
- Simple process easy manipulation
- The time to perform PCR is fast, only about 1 hour and 30 minutes
- High specificity and sensitivity
- Taqman probe technology
TopSENSI® GBS qPCR KIT
SPECIFICATIONS
| Target | Group B Streptococcus (GBS) |
| Input sample | Sample for vaginal or rectal swab |
| Input sample volume | 200µL |
| DNA volume | 10µL |
| Color channel detection | FAM: GBS detectionHEX: internal control |
| Sensitivity | 15,00 copies/ reaction |
| Technology | TaqMan probe |
| PCR time | 1h30m |
| Specificity | Only detect GBS |
| Component | GBS qPCR mix, negative control, positive control, internal control, tube PCR |
| Storage | 12 months at -20oC |
Recommended extraction kit
| Silica column extraction | HI-112
HI-004 |
TopPURE® Genomic DNA Extraction Kit |
| Automatic extraction | HI-612 | TopPURE® Maga Genomic DNA/RNA Extraction Kit |
