Introduction
TopPURE® Stool DNA Extraction Kit (HI-192) is manufactured based on silica column membrane technology according to ISO 9001 and ISO 13485 standards, allowing high quality DNA to be obtained.
- The kit is designed for total DNA extraction from fresh or frozen stool samples
- Simultaneous isolation of host DNA and microbial DNA
- Removal of PCR inhibitors in stool samples: Acid humic
- High-quality DNA ready to use in downstream applications
TopPURE® Stool DNA Extraction Kit
HIGHLIGHTS
- Quick and easy processing, high quality of extracted DNA in 45 minutes
- No organic extraction or alcohol precipitation
- The common process – simple column extraction
- High yield of DNA
- Removal of PCR inhibitors in stool samples: Acid humic
- High sensitivity in Realtime PCR
SPECIFICATIONS
- Input sample: fresh or freeze fecals
- Input sample volume: 200µL liquid or 200mg solid
- Elution buffer: 50 µL DNA/RNA
- Extraction time: 25 minutes
- OD: A260/A280 = 1.7 – 2.2
- Storage: Buffers can be stored dry at room temperature. Proteinase K stored at 2-8o
- Expiration date: 12 months from manufacture
COMPONENTS
| Component | Storage |
| TLX Buffer | Room temperature |
| CL buffer | |
| AMA Buffer | |
| WB1 Buffer | |
| WB2 Buffer | |
| EB Buffer | |
| TE Buffer | |
| Ethanol | |
| Silica column | |
| Tube 1.5 ml | |
| Proteinase K | 2-8oC |
DATA EXPERIMENT
Figure: Real-time PCR survey with GBS samples that extracted with HI-192.
