INTRODUCTION
TopPURE® Serum Viral RNA Extraction Kit is manufactured based on silica column membrane technology according to ISO 9001 and ISO 13485 standards, allowing high quality RNA to be obtained. This kit is designed for viral RNA extraction from serum, plasma, suspension, surface swabs, fluid smear (medical), negative-gram cultured cells and bacteria.
TopPURE® Serum Viral RNA Extraction Kit
HIGHLIGHTS
- Quick and easy processing, high-quality DNA extraction
- High purified RNA from various input samples
- Exclusion of hazardous chemicals like Phenol and Chloroform, ensuring safety in medical applications.
- Convenient: Provide a complete all chemical without needing to add chemicals or materials outside.
- The kit includes Ethanol for mixing Wash Buffer 1 and Wash Buffer 2
SPECIFICATIONS
- Input sample types: Serum/plasma, gram (-) bacteria
- Input sample volume: 300 µL (Serum, plasma)/ 200 µL (remaining samples)
- Collection volume: 50 µL RNA
- Time-saving: 30 minutes /10 samples
- OD: A260/A280 = 1.7 – 2.2
- Storage conditions: Room temperature. Except for Proteinase K and Carrier RNA stored at 2-8oC.
- Expiration date: 12 months from manufacture
COMPONENTS
| Component | Storage |
| RL Buffer |
Room temperature |
| WB1 Buffer | |
| WB2 Buffer | |
| EB Buffer | |
| Ethanol | |
| Silica column | |
| Tube 1.5mL | |
| DEPC | |
| Proteinase K | 2 – 8oC |
| RNA Carrier |
DATA EXPERIMENT
Figure 1. The comparison of silica column extraction HI-212 kit on whole blood samples between ABT (Red) and other foreign supplier N (Blue) on MrNV sample.
Figure 2. The 10% suspension sample (shrimp) was confirmed positive for MrNV, then diluted 10-fold with PBS Buffer. RNA was extracted using a HI-212 silica column extraction kit. Each dilution concentration was repeated 3 times.
