INTRODUCTION
TopPURE® PLASMID DNA EXTRACTION KIT provides a convenient and flexible solution for obtaining purified plasmid DNA from bacteria using the silica column method. This extraction kit combines an efficient lysis buffer system including PS, PL, and PP, with a simple, safe, and fast procedure, taking less than 30 minutes. The results of obtaining purified plasmid DNA from bacteria can be directly applied in downstream experiments.
TopPURE® PLASMID DNA EXTRACTION KIT
HIGHLIGHTS
- Simple and fast procedure
- High quality purification
- No organic solvent or toxic reagent (Phenol, Chloroform)
- Convenient: Provide a complete all chemical without needing to add chemicals or materials outside.
- The kit includes Ethanol for mixing Wash Buffer 1 and Wash Buffer 2
SPECIFICATIONS
- Input sample types: plasmid DNA of bacteria
- Input samples: 0.5 – 4mL bacterial cultured
- Receiving volume: 100 µL DNA.
- Time-saving: 30 minutes /10 samples
- High purification: A260/A280 = 1.7 – 2.1
- Storage: Spin columns and buffers can be stored dry at room temperature. RNase A stored at -20oC)
- Expiration date: 12 months from manufacture
COMPONENTS
| Component | Storage |
| PS Buffer | Room temperature |
| PL Buffer | |
| PP Buffer | |
| WB1 Buffer | |
| WB2 Buffer | |
| EB Buffer | |
| Ethanol | |
| 1.5 mL Tube | |
| Silica column | |
| RNase A | -20oC |
DATA EXPERIMENT
