INTRODUCTION
TopPURE® Maga Serum DNA Extraction Kit is manufactured based on automated magnetic bead-based extraction technology, meeting ISO 9001 and ISO 13485 quality standards. This kit provides an optimized solution in terms of time, cost-effectiveness, and efficiency for DNA extraction from diverse input sample types, such as serum, plasma, suspension, surface swabs, medical smears, and gram-negative bacterial cells.
HIGHLIGHTS
- Fast and efficient automatic extraction process from a variety of input sample matrices
- High DNA collection efficiency and purity
- Exclusion of hazardous chemicals like Phenol and Chloroform, ensuring safety in medical applications.
- Convenience: The kit comes in undivided chemical form, giving customers flexibility in use. Save chemicals with small sample quantities.
- The kit provides a complete set without the need for additional external chemicals or supplies, including Ethanol to mix Wash Buffer 1 and Wash Buffer 2.
- The kit can be used on a variety of different automatic extraction machines 32 and 96 in the markets
SPECIFICATIONS
- Input samples: Serum, plasma, suspension (suspension), surface scan samples, smear samples (medical), gram-negative bacterial cell samples
- Input sample volume: 200 µL
- Receiving volume: 100 µL DNA.
- Extraction time: 30 minutes for 1-96 samples
- OD: A260/A280 = 1.7 – 2.2
- Storage conditions: Room temperature. Except for Proteinase K, Magbeads and Carrier RNA stored at 2-8oC.
- Expiration date: 12 months from manufacture
COMPONENTS
| Components | Unit |
| NL Buffer | 2×30 mL |
| WB1 Buffer * | 1×36 mL |
| WB2 Buffer * | 2×18 mL |
| EB Buffer | 2×15 mL |
| Proteinase K | 2×1.2 mL |
| Magbead | 4×1.3 mL |
| Ethanol | 1×120 mL |
| 2 mL Deep Plate | 6 pcs |
| Tip Comb | 1 pcs |
| RNA carrier | 2 tubes |
| DEPC water | 2*1 |
DATA EXPERIMENT
Figure 1: Real-time PCR result in serum sample includes 10³ copies/mL (HBV virus). The sample was extracted 3 times/day using HI-002 kit on Bioer NPA-32 system.
Figure 2: Results comparing ABT’s HI-002 magnetic extraction kit on HBV samples with another foreign supplier.
