INTRODUCTION
TopPURE® Food DNA Extraction Kit (HI-202) is manufactured based on silica column membrane technology according to ISO 9001 and ISO 13485 standards, allowing high quality DNA to be obtained. This kit is designed for DNA extraction from raw food samples, processed foods, animal feed, nuts, etc.
TopPURE® Food DNA Extraction Kit
HIGHLIGHTS
- Quick and easy processing, high-quality DNA extraction within 35 minutes (10 samples)
- No organic extraction or alcohol precipitation
- The common process – simple column extraction
- High yield of DNA
- Removal of PCR inhibitors in stool samples: Acid humic
- High sensitivity in using Real-time PCR
SPECIFICATIONS
- Iput sample: raw food samples, processed foods, animal feed, nuts, etc.
- Input sample volume: 200mg solid
- Extraction time: 35 minutes
- OD: A260/A280 = 1.7 – 2.2
- Storage: Buffers can be stored dry at room temperature. Proteinase K and RNA Carrier are stored at 2-8oC
- Expiration date: 12 months from manufacture
COMPONENTS
| Component | Storage |
| FLB Buffer | Room temperature |
| PRB Buffer | |
| FTC Buffer | |
| WB2 Buffer | |
| EB Buffer | |
| Ethanol | |
| Silica column | |
| Tube 1.5 ml | |
| Proteinase K | |
| Note before use: – (*) Add ethanol to WB2 buffer for the first time according to the instructions on the bottle label. – Buffer FTC contains Chloroform, need to use equipment when handling. |
|
| Storage | Buffers can be stored dry at room temperature. Proteinase K stored at 2-8oC |
DATA EXPERIMENT
Figure: Real-time PCR survey with WSSV-infected shrimp meat samples.
