INTRODUCTION
FFPE is a cancer biopsy tissue sample, fixed with formalin and molded paraffin to help preserve tissue structure intact for a long time. This is also a great resource for retrospective research on cancer, histopathology, and molecular genetics. Currently, gene sequencing, Realtime PCR are often used to accurately detect gene mutations, quantify cancer gene expression, etc. Therefore, DNA extraction from FFPE tissue is an important first step.
TopPURE® FFPE DNA Extraction Kit is based on silica column technology according to ISO 9001 and ISO 13485 standards which allows to obtain high quality shrimps.
TopPURE® FFPE DNA Extraction Kit
HIGHLIGHTS
- Safety – Solvents are not toxic
- The quality of the resulting DNA has a high integrity, helping to increase PCR amplification of large segments.
- Follow to silica column principle: easy, convenient
- Efficiency: able to collect 12ug
- Completed solution: include Paraffin removal step and DNA extraction
SPECIFICATIONS
- Sample type: FFPE
- Input sample volume: 8 samples with 5-10µm thick
- Efficiency: 12µg
- Extraction time: 3hours
- OD: A260/A280 = 1.7 – 2.2
- Storage: Buffers can be stored dry at room temperature. Proteinase K stored at 2-8oC
- Expiration date: 12 months from manufacture
COMPONENTS
| Component | Storage |
| Mineral oil | Room temperature |
| TL Buffer | |
| CL buffer | |
| WB1 Buffer | |
| WB2 Buffer | |
| EB Buffer | |
| Ethanol | |
| Silica column | |
| Tube 1.5 ml | |
| Proteinase K | 2-8oC |
DATA EXPERIMENT
Figure 1: PCR results of extracted products for b-actin gene with different sized fragments.
