INTRODUCTION
TopPURE® BLOOD DNA EXTRACTION KIT is manufactured based on silica column membrane technology according to ISO 9001 and ISO 13485 standards, allowing high-quality DNA collection from whole blood samples containing anticoagulants (EDTA, citrate). The kit is designed with a simple, fast, and safe process in mind. The results of obtaining purified DNA are applied directly in experiments: Real-time PCR, sequencing, southern blot, SNP,…
TopPURE® BLOOD DNA EXTRACTION KIT
HIGHLIGHTS
- Process for rapid extraction of whole blood samples from humans or animals
- High quality purification
- No organic solvent or toxic reagent (Phenol, Chloroform)
- Convenient: Provide a complete all chemical without needing to add chemicals or materials outside.
- The kit includes Ethanol for mixing Wash Buffer 1 and Wash Buffer 2
SPECIFICATIONS
- Input sample types: gDNA or viral DNA from animal/human whole blood
- Input sample volume: 200 µL
- Elution buffer: 50 µL DNA
- Time-saving: 30 minutes /10 samples
- OD: A260/A280 = 1.7 – 2.2
- Storage: Spin columns and buffers can be stored dry at room temperature. Proteinase K stored at 2-80C).
- Expiration date: 12 months from manufacture
COMPONENTS
| Component | Storage |
| BL Buffer | Room temperature |
| WB1 Buffer | |
| WB2 Buffer | |
| EB Buffer | |
| Ethanol | |
| 1.5 mL Tube | |
| Silica column | |
| Proteinase K | -20oC |
DATA EXPERIMENT
Figure 1: The comparison of silica column extraction kit on whole blood samples between HI-132 of ABT (Green) and other foreign suppliers (Red, Blue)
Figure 2: Electrophoresis results of the DNA genome from Whole blood samples by EDTA (1), Citrate (2), Heparin (3), Balance (4)
