nQ16-X4/5

Ultra-Fast and Precise thermal cycling

● High-efficiency gold-plated Peltier  provides heating and cooling rate up to 10°C/s, contribute to a 45 cycle amplification within 25 min.

● Innovative alloy block design provides high temperature uniformity of ±0.15°C.

● With the specially designed cooling system, it can maintain high-speed heating and cooling with an average operating noise of 50 dB.

Up to 10-log dynamic range

Results on the nQ16-X4/5 System show excellent reproducibility and resolution down to very low copy numbers.

 

Innovative Optic Design

● Based on confocal scanning fluorescence detection technology, it can effectively remove the interference of background light and excitation light; obtain fluorescent signals with a high signal-to-noise ratio, and save the use of fluorescent probes and dyes. Additionally, ROX calibration is not required.

● High-performance silicon PMT can obtain high fluorescence signals under weak excitation light and reduce photobleaching.

 

Excellent Reproducibility

● Robust design ensures superior uniformity from run to run. Average quantification cycle(Cq)=17.8±0.10.

 

Melting Curve Analysis

● 16 replicates of human genomic DNA were amplified using SYBR.  The reactions were run under fast run mode, showing thermal uniformity as measured by the derivative peak with a melting temperature (Tm) of 77.5°C (standard deviation 0.05°C).

 

High Resolution to Detect 1.33-Fold

● The excellent uniformity of temperature together with quick detection of data guarantee the reaction temperature and time to be identical between different tubes, so it can reliably detect 1.33-fold differences in target amount.

● The figure shows the curve of 4 gradient dilutions: 2.25C, 1.5C,1.33C,1C, each concentration has 4 replicates.

Ultra-Fast Mode

● Benefiting from the innovative data gathering design and high-speed temperature control system, the detection time of the 5-colors and 16-wells can be shortened to 2seconds, which can minimize the difference in PCR extension time of different wells caused by the detection time. The experiment with 40 cycles can be completed within 20 minutes. The yellow curve was Standard speed (60minutes for 40Cycles) and the blue curve was Fast mode (25minutes for 40Cycles)

Multi Melting Curve Analysis

● The system enables the analysis of up to 5 target genes in one reaction with Multi Melting Curve Analysis. The melting temperature range from 40℃-95℃, with 0.5℃ resolution in all the fluorescence channels, and can provide over 4×5 target in a single tube reaction.

User-friendly Software Interface Design

● The software of uses a navigational operation interface, which is intuitive and easy to use.

● The unique project management design help users efficiently manage common detection items and quickly complete the experimental setup. The user can create a project including the experimental setting (experiment type, detection target, PCR reaction protocol, etc.) information. Before the experiment, the user can select the existing project and only needs to set the reaction well position to quick start the run. Moreover, it supports one-click sample information Import to shorten the time to setup sample information.

● The equipment is equipped with Bluetooth connectivity, which supports remote connection without the need for data cables, making it convenient to be placed in a biosafety cabinet for safe and pollution-free operations