1. Introducing the 16-sample automatic nucleic acid extraction machine
ABT 16-sample automatic nucleic acid extraction machine (EZ16) uses magnetic characteristics of nucleic acids to develop a rapid and synchronous DNA/RNA extraction process. Extraction technology by Magnetism helps save time in DNA/RNA extraction and purification steps for a variety of sample volumes from 1-16 samples in one run.
The capacity of the ABT extraction machine can be up to 16 samples per run for 30 minutes. In addition to standard consumables used to extract maximum sample volumes, ABT also provides consumables used to operate the extraction machine with small sample volumes, helping to save costs on chemicals and accompanying supplies. (Refer to the introduction video)
2. Device characteristics and applications.
EZ16 is safe and reliable, can be used to extract DNA, RNA from whole blood samples, cells, tissues and other sample types (depending on the extraction kit), saving time and money. effort. The extracted product can be used directly for downstream techniques. Some features of the EZ16 model 16 separator:
- Mechanism of action: Magnetic bar stamps the sample
- Uniform magnetic bar design: Ensures no remaining magnetic particles, suitable for all small sized magnetic particles.
- UV disinfection light system: Minimizes the risk of foreign contamination.
- Flexible Programs: Multiple programs can be edited, changed and cached, ensuring high consistency and repeatability.
- Friendly user interface: The control panel displays running programs and parameters simultaneously, easy to use.
- Temperature control: Automatic heating system enhances the efficiency of lysis and elution.
Principle of operation:
Using magnetic particle separation technology, in which DNA/RNA is extracted from the sample solution mixture using magnetic force. At certain salt concentrations, nucleic acids bind to magnetic particles. A magnet is then used to create a strong magnetic field inside the solution. Magnetic beads bound to nucleic acids are attracted to the magnetic field and then the DNA or RNA is eluted with appropriate chemicals.
The steps of the extraction process include:
3. Operating conditions
Operating temperature: 10oC ~ 40oC
Operating humidity: 10% – 90%
Power source: AC 220V±10%
Attention :Before power on, please confirm whether the working conditions meet the above requirements. Pay special attention to the power line is reliably grounded.
4. Transportation and Storage Conditions
Ambient temperature: 25oC ~ 35oC
Environmental humidity: ≤85%.
5. Basic parameters
| Code | EZ16 |
| Sample size | 1-16 sample/run |
| Capacity | ~ 120W |
| Magnetic bead recovery efficiency | ≥ 98%. |
| Humidity | ≤ 85%. |
| Sample homogeneity | Cv<3% |
| Voltage | AC 220V±10% |
| Magnetic rod (fixed) | 2 × 8 pcs |
| Sample volume range per well | 50 ~ 1000μL |
| UV intensity | ≥ 40 µw/cm² |
| Most suitable size of magnetic beads | 0.2 ~ 1.0μm |
| Overall dimensions (mm) | 338 (L) x 352(W) x 415(H) |
| Net weight | 30Kg |
6. Consumable material solutions
For the EZ16 16-model separator, consumables include two solutions depending on sample run capacity:
6.1. Standard capacity: Used to run up to 16 samples/run simultaneously
– Deep-well plate Including 96 wells used for ABT extraction machine. The product is manufactured with high purity materials, sterilized and does not contain fine dust that contaminates the sample.
- Number of wells on the plate: 96 wells (8×12 wells)
- Well bottom shape: V shape
– Tipcomb is manufactured in plastic polypropylene (PP), used to cover magnetic rods for ABT separators. The product is suitable for handling magnetic beads due to their low binding affinity for nucleic acid molecules.
- Number of tips on tipcomb: 8 tips correspond to 8 magnetic bars
- Cylindrical shape with U-shaped sleeve bottom.
6.2. Optimal capacity: Used for sample runs with more than 1 sample/run
– Tipcomb is manufactured in plastic polypropylene (PP), used to cover magnetic bars for ABTseparators
– ABT® EZ32 Strips Tube is manufactured using high purity materials using a modern manufacturing process that ensures uniformity and accuracy high body. The product is sterilized and does not contain fine dust that contaminates the sample. The product can be used to replace 1.5mL tubes for sample storage and used in retail magnetic extraction.
– Rack from ABT® EZ32 Rack (ART-001) is made of sturdy stainless steel. The product is used as a base to support tube strips in magnetic extraction in ABTseparation machines.
*The above consumables can be used for the 16-model EZ16 separator, the 32-model EZ32 separator and some other Bioer models.
7. Extraction kit products used on ABT EZ16 machine
ABT optimizes its kits not only for ABT extraction machines but also to achieve high performance on most current extraction machines.
Refer to the kits below:
TopPURE® Maga Serum DNA/RNA Co-Extraction Kit (HI-007)
TopPURE ® Maga Genomic DNA/RNA Extraction Kit (HI-612)
TopPURE® Maga viral DNARNA Extraction Kit (HI-812)
TopPURE® Maga Quick Viral Extraction Kit (HI-912)
8. Notes when using the 16-sample extraction machine
– Ensure biological safety when performing tests
– Be sure to attach the Tipcomb before running to avoid damage to the model and machine
– When an incident occurs, clean the machine with alcohol and DNA/RNA Away
– Carefully take the sample and place the sample disc in the tray
– Avoid placing the sample plate near UV rays, sunlight or spending too long in the air
– Avoid removing alcohol from the sample plate
– Remove UV and clean with alcohol after each run
